Uveal Melanoma Sample Preparation

Impact Genetics provides sample collection kits upon request.

Please email info@impactgenetics.com or call 1-877-624-9769 to provide us your mailing address and quantity required.

Kit includes:

  • Required forms: Uveal Melanoma Informed Consent (1a), requisition form (1b) and payment forms (1c, 1d, 1e)
  • One tube with cell lysis for tumor samples
  • One tube with cell lysis for buccal swab samples
  • One sterile nylon bristle cytology brush for buccal swab collection
  • Plastic bag to place samples in
  • Absorbent pad for shipping
  • Rigid box for shipping
  • Patient labels
  • Purolator or FedEx shipping materials (4 copies of the Pro-Forma Invoice for shipment outside Canada)

Sample Requirements

Samples required (both required):

  • Normal sample: buccal swab or blood sample
  • Tumor sample: tumor biopsy (FNAB or other)

Sample Preparation Instructions
 
Normal sample:

    Blood samples for DNA:

  • 10 mls venous blood in yellow-topped ACD tubes or lavender-topped EDTA tubes at room temperature, to be received within 5 days after draw.
  •  
    Or
     
    Buccal swab:

  • Do a gentle mouth rinse with water to clear the mouth of debris.
  • Scrape the inside of the patient’s mouth using ten strokes with one of the collection brushes (supplied). Either cheek is fine.
  • Cut the handle from the collection brush using sterile scissors or a razor blade.
  • Place the brush in one of the collection tubes (supplied).
  • Label the tube with one of the stickers (supplied), complete with the patient name and date of birth.
  • Once the buccal sample is in the tube, seal tube with twist top cap, it can be stored at room temperature or refrigerated (4 ̊C or 39 ̊F) until shipping.
  • Place the tube in the plastic bag and rigid container (provided).

Tumor sample:

    FNAB:

  • Place the FNAB sample in the collection tube (supplied). Two or more FNAB passes are preferred and all passes can be combined in one tube.
  •  
    Or
     
    Biopsy:

  • If possible, larger tumor tissue samples from a biopsy are preferred. Place the fresh tumor sample in the collection tube (supplied). DO NOT FIX the tissue.
  • Label the tube with one of the stickers (supplied), complete with the patient name and date of birth.
  • Once the tumor sample is in the tube, seal tube with twist top cap. Tube can be refrigerated stored (4 ̊C or 39 ̊F) until
    shipping.
  • Place the tubes in the plastic bag and rigid container (provided). Leave the absorbent pad in the plastic bag.

 

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